Plasmid

Part:BBa_K3978015:Design

Designed by: Haozhen He   Group: iGEM21_HiZJU-China   (2021-10-21)


BL21 vector combined with amoA and hao gene sequence


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 811
    Illegal EcoRI site found at 2673
    Illegal XbaI site found at 2591
    Illegal PstI site found at 1552
    Illegal PstI site found at 2692
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 811
    Illegal EcoRI site found at 2673
    Illegal PstI site found at 1552
    Illegal PstI site found at 2692
    Illegal NotI site found at 2710
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 811
    Illegal EcoRI site found at 2673
    Illegal BglII site found at 2866
    Illegal BamHI site found at 246
    Illegal BamHI site found at 2667
    Illegal XhoI site found at 2915
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 811
    Illegal EcoRI site found at 2673
    Illegal XbaI site found at 2591
    Illegal PstI site found at 1552
    Illegal PstI site found at 2692
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 811
    Illegal EcoRI site found at 2673
    Illegal XbaI site found at 2591
    Illegal PstI site found at 1552
    Illegal PstI site found at 2692
    Illegal NgoMIV site found at 2885
    Illegal NgoMIV site found at 3232
    Illegal NgoMIV site found at 7909
    Illegal AgeI site found at 270
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 328
    Illegal BsaI.rc site found at 709
    Illegal BsaI.rc site found at 3817
    Illegal SapI.rc site found at 5477


Design Notes

this gene can be effectively transferred into BL21 by electrotransformation. Meanwhile IPTG have to be added in the expression system to induce the expression of protein.


Source

gene sequence BBa_K3978011 BBa_K3978040, lac operater, rbs B0030 terminator B0015

References